【摘要】 目的　研究雷公藤单体对胶质瘤细胞的体外抑制作用。方法　MTT法测定 3种雷公藤单体对胶质瘤细胞株SHG44、C6、U2 5 1的体外抑制作用 ;免疫组化法观察雷公藤甲素与雷公藤红素对SHG44胶质瘤细胞bax、bcl- 2蛋白表达的变化。结果　雷公藤二萜类单体雷公藤甲素对胶质瘤细胞有极明显的抑制作用 ;雷公藤三萜类单体雷公藤红素的抑制作用次之。两者均使SHG44细胞bax表达增加 ,bcl- 2表达下降。结论　雷公藤甲素与雷公藤红素对胶质瘤细胞有明显的抗肿瘤作用 ,其作用与促进bax表达 ,抑制bcl- 2表达 ,促进细胞凋亡有关更多还原

【Abstract】 Objective To investigate inhibitory effects of three monomes of Tripterygium wilfordii on the proliferation of glioma cells.Methods The effect of three monomes on the proliferation of glioma cells SHG44?C6?U251 in vitro was examined using MTT assay. The expression of bax and bcl-2 on SHG44 was measured with immunohistochemistry after treated with triptolide and celastrol.Results The proliferation of glioma cells was remarkable inhibited by triptolide. Celastrol took the second place.They both made the SHG44 cells increase the express of bax and decrease the express of bcl-2.Conclusion Triptolide and celastrol inhibit the proliferation of glioma cells in vitro. They promote the expression of bax,inhibit the express of bcl-2 and accelerate cell apotosis,which may be the mechanism of inhibition.更多还原