【摘要】 目的　评价新药苯丁酸钠对胶质瘤细胞的诱导分化作用 ,为胶质瘤诱导分化相关基因研究作前期准备。方法　采用MTT法、软琼脂克隆形成率、形态学、流式细胞术、GFAP免疫荧光等方法鉴定分化程度 ,用划痕实验检测细胞运动能力的改变。结果　 2mM苯丁酸钠即可明显诱导人脑胶质瘤低分化细胞株SHG - 4 4 - 9的细胞分化 ,表现为增殖抑制 ,克隆形成能力丧失 ,细胞突起增长 ,G1期阻滞 ,GFAP表达量升高 ,细胞运动能力下降。结论　苯丁酸钠可以高效诱导人脑胶质瘤细胞分化 ,并使细胞运动能力下降 ,有可能发生分化相关基因表达改变更多还原

【Abstract】 Objective To measure the differentiation inducing effect of Sodium phenylbutyrate,a novel inducer,on human glioma cells,prepared for further study of induced glioma cell differentiation associated genes.Methods The differentiation of human glioma cell strain SHG-44-9 induced by sodium phenylbutyrate was confirmed by 5 tests,such as MTT,soft agar clone forming test,flow cytometry,GFAP immunofluorescency and morphology,furthermore, cell mobility was studied by scratch test.Results The glioma cells were induced to differentiation by 2mM sodium phenylbutyrate obviously,showing growth arrest,clone-forming ability loss,extension of cell processes,G 1 phase cells accumulating,GFAP expression increasing and mobility inhibition.Conclusion Human glioma cells could be induced differentiation by sodium phenylbutyrate sensitively,with mobility inhibited and the expression of genes associated with glioma cell differentiation may change.更多还原