【摘要】 目的　了解乙型肝炎患者 HBe Ag和 HBe Ab双阳性状态下 C基因启动子区 ( BCP)变异情况。方法　采用时间分辨荧光免疫分析方法定量检测乙型肝炎“二对半”,对 HBe Ag/ HBe Ab双阳性标本采用巢式聚合酶链反应扩增目的 DNA片段 ,并对阳性的 PCR产物直接标记测序 ,测序结果和 Grenbank中登录的标准序列相比较。结果　对 17例 HBe Ag和 HBe Ab双阳性患者血清中 HBV DNA进行了检测 ,阳性 13例 ,测序结果显示 ,13例 HBV DNA阳性者均存在 T1762和 A1764的突变。结论　在乙型肝炎 HBe转换过程中均伴有 C基因启动子区 T1762与 A1764变异更多还原

【Abstract】 Objective To investigate the mutations of hepatitis B virus (HBV) core promoter gene isolated from both HBeAg and HBeAb positive patients.Methods HBV markers were detected by dissociation enhanced lanthanide fluoroimmunoassay (DELFIA),HBV core promoter gene was amplified by polymerase chain reaction and sequenced directly in ABI 310 machine.The core promoter gene sequences were compared with the representative sequences in Genbank,then the mutations were found.Results 13 of 17 patients were positive for HBV DNA;the sequence analysis showed that all sequences had mutations.T1762 and A1764 mutations were found in all patients.Conclusion T1762 and A1764 can be detected in all patients who had HBe seroconversion更多还原