【摘要】 目的　研究不同粒径大气颗粒有机提取物对人群健康的影响。方法　使用DFJ - 1型五段分级采样器采集太原市某一居民区的大气颗粒物 ,分别用二氯甲烷、丙酮、甲醇经索氏提取器获得不同粒径颗粒物的有机提取物 ,再用多核细胞法 (CB)测定其致人淋巴细胞HPRT位点突变频率 (MF)。结果　大气颗粒物中有机提取物含量随粒径减小而增加。不同粒径有机提取物致HPRT基因位点突变 ,除粒径范围≥ 7.0 μm的最低剂量组 (2 5mg/L)与阴性对照组比较无显著差异外 (P >0 .0 5) ,其他各层各剂量组均较阴性对照组明显增高 (均P <0 .0 1 ) ,且随着粒径减小 ,MF增高。结论　HPRT基因位点对不同粒径大气颗粒有机提取物致突变作用非常敏感 ,有明显的剂量效应关系更多还原

【Abstract】 Objective To study the effect of organic extracts of air particles with various diameters on human health. Methods Using a five stage sampling scheme, airborne particles were collected in a residential area of Taiyuan city.Organic particles were extracted by dichloromethane, acetone and methanol, then the HPRT gene locus mutation frequency(MF) was examined by multinucleus cell assay. Results The particle size in organic extracts content less than 1.1 μm was the biggest among different diameter airborne particles. MF increased with decrease of diameter. There was no significant difference on HPRT gene locus mutation between the group more than 7.0 μm in dose of 25 mg/L and the control group ( P >0.05), while the mutation rate of other dose groups showed significantly higher than that of control group ( P <0 01). A good linear relationship between HPRT gene mutation frequency and dose was observed. Conclusion HPRT gene locus is very sensitive to mutagenesis from airborne particles and has a good dose response relationship.更多还原