【摘要】 在 0℃、p H7.4条件下 ,使用 1 2 5I标记人血清脱铁转铁蛋白法测定了双 ( )转铁蛋白 (Tb C- 1 2 5I- apo Tf-Tb N)与 K5 6 2细胞的结合。结果表明 Tb C- 1 2 5I- apo Tf- Tb N 与 K5 6 2细胞结合的平衡常数为 2 .7× 10 7mol- 1· L,K5 6 2细胞的转铁蛋白受体数为 1.0× 10 6。更多还原

【Abstract】 Human serum apotransferrins were labeled by using sodium iodide-125.In 0.1 mol/L N-(2-hydroxyethyl)piperazine-N′-2-ethanesulfonic acid at 0 ℃ and pH 7.4 the binding of diterbium(Ⅲ) human serum transferrin to the surface of K562 cells,a human leukemic cell line,was studied.The results show that the binding constant for diterbium(Ⅲ) transferrin with K562 cell is 2.7×10 7mol -1·L and the number of binding sites on the surface is 1.0×10 6.更多还原