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山东省根瘤菌表型多样性及数值分析

Diversity of phenotypic features and numerical analysis of Rhizobia isola-ted from Shandong Province

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【作者】 韦革宏郝鲜俊孙科技刘虎岐陈文新

【Author】 WEI Ge-Hong1, HAO Xian-Jun1, SUN Ke-Ji1,LIU Hu-Qi2, CHEN Wen-Xin31 College of Resources and Environment, Northwest Sci-Tech University of Agriculture and Forestry, Yan-gling, Shaanxi 7121002 College of Life Sciences, Northwest Sci-Tech University of Agric

【机构】 西北农林科技大学资源环境学院西北农林科技大学生命科学学院中国农业大学生物学院 陕西杨凌712100陕西杨凌712100北京100094

【摘要】 在山东省根瘤菌资源调查的基础上,选取31株快生根瘤菌和34株参比菌株,进行了102项表型性状测定和聚类分析。结果表明:不同地理来源、甚至同一地区来源或同种寄主的不同菌株在碳氮源利用、抗生素抗性、耐逆性等方面存在着较高的多样性。32%的菌株可耐受300μg/mL的新霉素,39%的菌株可耐受300 μg/mL的青霉素和洁霉素。2株菌能耐受4.0%的NaCl。13株菌可以在pH11的条件下生长。在87%的相似水平上,未知菌株形成2个独立的亚群,第1亚群有3株菌,中心菌株为 SD109;第2亚群有 4株菌,中心菌株为 SD083。

【Abstract】 Based on investigation of rhizobial resources of Shandong Province, 102 phenotypic features of31 fast-growing rhizobia strains and 34 reference strains were analyzed and numerically classified. The re-sults revealed huge diversity among strains isolated from different places. Strains with the same geographicresources and the same hosts differed in carbon and nitrogen nutrient utilization, resistance to antibiotics,and tolerance to salt, alkali and chemical dye. Thirty two percent of strains could resist 300 μg/mL Neo-mycin and 39 percent of strains could resist 300 μg/mL Penicillin and Jiemycin. Two strains could growin medium with 4 % NaCl and 13 strains grew in pH 11 medium. Unknown strains were clustered intotwo new subgroups different from known species at the similarity level of 87%. Cluster 1 had threestrains, of which SD109 was the central strain. Cluster 2 had four strains, and SD083 was the centralstrain.

【关键词】 根瘤菌表型性状多样性山东省
【Key words】 Rhizobiaphenotypic featurediversityShandong Province
【基金】 国家自然科学基金重点项目(39730010);国家自然科学基金项目 (30000005)
  • 【文献出处】 生物多样性 ,Chinese Biodiversity , 编辑部邮箱 ,2002年02期
  • 【分类号】Q939.114
  • 【被引频次】5
  • 【下载频次】170
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