【摘要】 在空气氧化青霉素衍生物合成亚砜的反应中 ,用溶胶 凝胶法包容催化剂乙酰丙酮钴 (Ⅲ ) ,使反应在非均相条件下进行。采用反相高效液相色谱法测定了该反应的转化率。采用的色谱柱为C1 8反相柱 (4 6mmi d × 1 50mm ,1 0 μm) ,流动相为甲醇 水 (体积比为 85∶1 5)溶液。在 2 70nm检测波长下 ,青霉素G 对甲氧基苄基酯亚砜(PGPMBO)检测量在 0 0 5g·L- 1 ～ 0 40g·L- 1 时 ,与其峰面积具有良好的线性关系 ,线性相关系数为 0 9992。在不同反应条件下反应的最高转化率为 98 8%。该方法可直接对反应体系进行测定 ,可以快速准确地测定出反应的转化率 ,准确地反映出反应的真实情况更多还原

【Abstract】 In air oxidation of penicillin G p methoxybenzyl ester (PGPMB) to its sulfoxides (PGPMBO), sol gel technique was employed to encapsulate the catalyst Co(acac) 3, by which the reaction was run under heterogeneous conditions. A reversed phase high performance liquid chromatographic method was established for the determination of the conversion of this reaction. The analysis of PGPMBO was carried out on a C18 column (4 6 mm i d ×150 mm, 10 μm) with the mobile phase of methanol water (85∶15, V/V ), at a flow rate of 1 mL·min -1 and with detection wavelength of 270 nm The linearity of the calibration curve of PGPMBO was obtained over the range of 0 05 g·L -1 -0 40 g·L -1 with a relative coefficient of 0 999?2 The highest conversion was 98 8% The method can be applied to determine this conversion promptly in the reaction system更多还原