【摘要】 建立了快速分离和检测引起仔猪腹泻的肠毒性大肠杆菌K88、K99和 987P细菌细胞的毛细管区带电泳方法 ,并进行了腹泻仔猪粪便中肠毒性大肠杆菌的应用检测分析。结果表明 ,在电泳缓冲液为 0 0 5mol/LNa2 CO3 NaHCO3(pH 9 9)、分离电压为 1 4 1kV、检测波长为 2 1 0nm的电泳条件下 ,E coliK88、K99和 987P的细胞分别具有单一、稳定的特征谱峰 ,其保留时间的相对标准偏差RSD≤ 0 9% ;在确定的实验条件下 ,实现了腹泻仔猪粪便中肠毒性大肠杆菌的快速检测分析 ,发现将出生 5d～ 6d的腹泻仔猪粪便引入培养基中增殖后主要检出K88,将出生 30d～ 35d的腹泻仔猪粪便引入培养基中增殖后主要检出K99,将出生 60d左右的腹泻仔猪粪便引入培养基中增殖后未发现肠毒性大肠杆菌。更多还原

【Abstract】 Rapid separation and determination of enterotoxigenic E coli K88, K99 and 987P with intact cells using capillary zone electrophoresis (CZE) are described. The CZE was performed in a running buffer of 0 05 mol/L Na 2CO 3 NaHCO 3 (pH 9 9), under applied voltage of 14 1 kV and detected by an on line UV at 210 nm. Results show that K88, K99 and 987P were separated completely. Moreover, each strain presented a characteristic peak and a reproducible retention time (RSD≤ 0 9% ). The successful analysis of enterotoxigenic E coli in the excrement of diarrhea piglets is also discussed. K88 and K99 were detected in the excrement of diarrhea piglets born in 5 d-6 d, and 30 d-35 d respectively, and nothing was detected in the excrement of diarrhea piglets born in about 60 d.更多还原