【摘要】 以酪氨酸为印迹分子 ,甲基丙烯酸为功能单体 ,三羟甲基丙烷三甲基丙烯酸酯 (TRIM )为交联剂 ,采用种子溶胀悬浮聚合法在水溶液中制备了一系列分子印迹聚合物微球 (MIPMs)。利用扫描电镜 (SEM)对此微球的粒径大小、粒径分布、表面孔与孔径分布等进行了分析研究 ,探讨了影响其形貌的主要因素 ,并将所得微球用作色谱固定相研究了其分子选择吸附性能。研究表明 ,种子溶胀悬浮聚合法能够制得单分散性较好的、表面带有微孔的分子印迹聚合物微球 ,且该微球呈现出较好的特异吸附性能。更多还原

【Abstract】 A series of molecularly imprinted polymeric microspheres(MIPMs) were prepared by seed swelling and suspension polymerization method in aqueous system using tyrosine as printing molecules, methacrylic acid as functional monomers and trimethylolpropane trimethacrylate (TRIM) as cross-linkers. The morphology including the size, size distribution, pore and pore distribution of the polymer beads was analyzed by scanning electron microscope(SEM). The major factors that influence these properties of the beads are discussed. The molecule selecting property of the MIPMs was detected through liquid chromatography.The results showed that uniform-sized spherical MIPMs had been prepared in aqueous system by seed swelling and suspension polymerization method, and its surface was distributed with pores between 1 μm to 10 μm. The average bead size was adjusted from about 50 μm to 400 μm by changing the stirring speed and the amount of seeds used during the preparation. The prepared MIPMs exhibited a considerable tendency to adsorb tyrosine, the printing molecules selectively, and the separation factor was up to 1.82 when phenylalanine was chosen as the competitive molecules.更多还原