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磺基醋酸汞试剂法用于C21甾苷定量检测的可行性研究

Fessibility Study on Method of C21 Steroidal Glycosides’ Quantitative Determination with Sulfonic Mercuric Acetate Reagent

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【摘要】 经实验确定了甾苷定量测定的官能团反应方法。用定量测定3-羟基族化合物的最特效的方法磺基醋酸汞试剂法直接测定C21甾苷元的C3-OH,从而间接测定C21甾苷,实验证明该方法是可行的。

【Abstract】 In order to establish the quantitative determination method for C21 steroidal glycoside in Asclepiadaceae plants as homologous health food ,the functional group reaction method of C21 steroidal glycoside’s quantitative determination was studied in this paper after the research on their extraction method.The sulfonic mercuric acetate reagent method,which was unique to 3-hydrooxyl steroids,was used to determine the C3-OH on the aglycones directly in order to deternine the glycosides indirectly .The results showed that the method was feasible .

  • 【分类号】O656
  • 【被引频次】9
  • 【下载频次】121
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