节点文献
酪蛋白抗诱变水解物生化特性的研究
Biochemistry Study on Antimutagenic Casein Pepsin Hydrolysis
【摘要】 采用Ams鼠伤寒沙门氏菌实验研究酪蛋白及酪蛋白的胃蛋白酶的水解物的抗诱变性,采用超滤和等电点法分离水解物,证明水解产物比酪蛋白具有更强的抗诱变性,经快速液相色谱FPLC和质谱法分析表明具有抗诱变性短肽是由9到11个氨基酸构成的,分子量在1000到2000道尔顿范围内。
【Abstract】 Antimutageic activity of casein and its pepsin hydrolysis product were studied with Salmonella Ames test.The digestion products have Shown stronger antimutagenicity against sodium azide than casein.The hydrolyzed peptides formed were separated by ultrafiltration or isoelectroic precipitation,and were characterized by FPLC and Mass spectra analysis.The peptides molecular weigh range was between to 1000 to 2000 dalton.and contained 9 to 11 amino acids.
【关键词】 酪蛋白;
胃蛋白酶;
快速液相色谱(FPLC);
质谱;
抗诱变性;
【Key words】 Casein Antimutagenicity Pepsin FPLC Mass spectra;
【Key words】 Casein Antimutagenicity Pepsin FPLC Mass spectra;
【基金】 天津市教委资助项目(990902)
- 【文献出处】 食品科学 ,Food Science , 编辑部邮箱 ,2002年09期
- 【分类号】R73-36
- 【被引频次】3
- 【下载频次】58