【摘要】 通过正交试验,确定了大肠杆菌E881产酶的最佳培养基为:葡萄糖(1.5%)酵母膏(1%)KH2PO4(0.3%)(NH4)2SO4(0.2%)。以此培养基培养出来的游离细胞为酶源,在底物浓度为0.2mol/L时进行转氨反应,反应6h,底物摩尔转化率达到90%以上。更多还原

【Abstract】 The medium for culture and productio n of L -Phenylalanine Transaminase o f Escherichia coli 881was optimized by orthogonal experiment s.The experiment showed that the opt imal medium ingredients were:gluco se(1.5%),yeast extract(1%),KH 2 PO 4 (0.3%)and(NH 4 ) 2 SO 4 (0.2%).With the free cell as enzyme source,the trans -ratio of phenylpyruvic acid was above 90%while substr ate concentration was 0.2mol /L and transaminase reaction time 6hours.更多还原