【摘要】 本试验采用宰后向牛的股二头肌注射CaCl2溶液和木瓜蛋白酶来嫩化牛肉,并于后熟24h左右进行了肌原纤维小片化指数(MFI)、粗钙激活因子活性(CAF)和可溶性胶原蛋白含量等指标的测定。实验结果表明,宰后注射CaCl2溶液和木瓜蛋白酶,显著降低了股二头肌的剪切力。注射3%CaCl2溶液导致CAF活性比对照组显著增强。试样的可溶性胶原蛋白含量与剪切力相关性显著(r=-0.96)。对照组MFI与相应的剪切力相关显著(r=-0.736)。因此,MFI可作为衡量常规方法后熟或注射CaCl2牛肉的嫩度的方法。对于采用注射植物蛋白酶嫩化的牛肉,测量其可溶性胶原蛋白含量对于预测嫩度具有重要意义。而CAF活性的测定则可衡量常规方法后熟或注射CaCl2溶液的牛肉后熟的程度及其嫩度。更多还原

【Abstract】 The solutioncomposedofpapainandCaCl 2 was injectedinprerigor into the hot boning femoralbiceps(FB)fromone side of the carcasses.The treated carcasse s were allowed to be aged for 24hrs.The activity of Ca 2+ -activate -factor(CAF),myofibril frag-mentationindex(MFI )andthe percentage of total intramuscular collagen solubilized by heati ng in1/4-strengthRinger solution were measuredonbothsides.Itrevealedt hatinjecting papainandCaCl 2 decreasedsignificantly.Increasing the activity ofCAFwithinjection of3%CaCl 2 resultinginalittlehigheractivityofCAFwithinjectionof0.3%CaCl 2 ornoinjectionofCaCl 2 ,as comparedtothecontrolts.更多还原