【摘要】 由固定化蔗糖转化酶(INV),葡萄糖变旋酶(MUT)及葡萄糖氧化酶(GOD)的复合酶膜组成的过氧化氢双电极系统,可同时测定样品中蔗糖和葡萄糖的含量,蔗糖电极的葡萄糖干扰用差分法消除。每次进样量25μl,响应时间30s,蔗糖在0～400mg/dl,葡萄糖在0～200mg/dl范围内具有良好的线性关系,连续测定20次蔗糖和葡萄糖的变异系统分别为1.13%和1.05%。蔗糖测定回收率为99.62%～100.97%,蔗糖酶膜使用寿命半个月。更多还原

【Abstract】 Thisbiosensorconsistedoftwoimmo bilizedenzymeelectrodes,oneofwhichwasglucoseoxidaseforglucose detection and the other was an integrated membrane of invertase,mutarotase and glucose oxidase for sucrose d etection.Glucose and sucrose could be determined simultaneously with injec tion amount 25μl and response time 30sec-onds.The biosensor showedgoodlinearity among the range of0～400mg /dl ofsucrose and0～200mg /dl ofglucose.The relative response deviationofsucrose andglucose was 1.13%and1.05%respectively in20assays.The reco very rate of sucrose was 99.62～100.97%.The membrane could be usedcontinuously for two weeks.更多还原