改善大豆分离蛋白溶解性的试验

【摘要】 采用木瓜蛋白酶对大豆分离蛋白进行了酶法改性试验。试验表明,酶法水解能显著提高大豆分离蛋白的溶解性。酶解的最佳条件是,pH7.0、温度55℃、底物浓度3％、酶的浓度12000u/g大豆分离蛋白。在此条件下酶解3h,大豆分离蛋白的水解度(DH)超过25％。即使在大豆蛋白的等电点,大豆分离蛋白酶解液仍然有较好的溶解性。更多还原

【Abstract】 Modification of soybean protein isolate with papain is studied. The results show that enzymatic hydrolysis improves solubility of soybean protein isolate obviously. The optimum conditions of enzymatic hydrolysis are pH: 7.0, hydrolysing temperature: 55, enzyme concentration: 12000u/g,concentration of substrate:3% ,after being reacted 3h the degree of hydrolysis of soybean protein isolate can go up to 25% . Even at the isoelectric point of soybean protein, its enzymatic hydrolysis solution also gets good solubility .更多还原