枯草杆菌蛋白酶水解大豆分离蛋白的研究

【摘要】 采用枯草杆菌蛋白酶对大豆分离蛋白进行水解工艺条件的研究。通过单因素分析 ,正交试验 ,确定了枯草杆菌蛋白酶酶解大豆分离蛋白的最佳水解条件 ,即 pH7 0、温度45℃、酶浓度2000u/g大豆分离蛋白、底物浓度3 %。在此条件下水解6h ,水解度可达60 %以上更多还原

【Abstract】 Hydrolysis of soybean protein isolate with subillisin is studied. The optimum conditions have been found by mono-factor analysis and orthogonal test as follows, pH:7.0, hydrolysing temperature:45℃, enzyme concentration:2000u per gram of substrate, concentration of substrate: 3%. At this optimum condition, reacted 6 hours, the degree of hydrolysis of soybean protein isolate can go up to 60%.更多还原