【摘要】 在已筛选出产海藻糖合酶菌株———恶臭假单胞杆菌的基础上 ,研究了该菌株最佳发酵培养基。恶臭假单胞杆菌H76的最适碳源为麦芽糖和葡萄糖 ,最适氮源为蛋白胨和酵母粉 ,无机盐为MgSO4 ·7H2 O ;该菌株产酶最佳培养基配方为 :麦芽糖 3 % ,葡萄糖 3 % ,Mg SO4 7H2 O 0 2 % ,蛋白胨 2 % ,酵母粉 0 7%。更多还原

【Abstract】 Trehalose is a natural functional disaccharide It could be widely used in foodstuff,pharmaceuticals,cosmetic and agriculture Trehalose synthase can convert maltose into trehalose by the intermolecular transglucosylation And it could be used in the scale production of trehalose This study mainly discussed the fermentation medium of Pseudomonas putida H76,which can produce trehalose synthase The results showed that the best carbon source for Pseudomonas putida H76 is maltose and glucose,the best source for the strain is peptone and yeast extraction,the best inorganic source is MgSO 4·7H 2O The formula of culture medium was optimized as:maltose 3%,glucose 3%,peptone 2%,yeast extraction 0 7%,MgSO 4·7H 2O 0 2%更多还原