【摘要】 从腐烂苹果、青椒等材料中分离出 6 78株菌 ,得到 1株菌A6经鉴定为葡萄糖酸杆菌(Gluconobactersp ) ,其发酵液分离提取产物经红外、紫外光谱、质谱、核磁共振和圆二色谱鉴定确证为 2 ,5 二酮基 D 葡萄糖酸。该菌的发酵条件研究表明 ,在含 2 5 %葡萄糖的玉米浆碳酸钙培养基中 ,2 8℃发酵 7d可生成 2 ,5 二酮基 D 葡萄糖酸 172 0mg/mL ,摩尔转化率为5 2 0 3%。在耐糖性方面高于国内同类报道。更多还原

【Abstract】 Strain A6 was identified as a member of Gluconobacter sp. which was obtained from 678 strains of fruit, vegetable and soil samples. The fermentation product from strain A6 was confirmed as 2,5-diketo-D-gluconate by UV and infrared absorption spectra, FABMS, C13-NMR and CD spectrum respectively. Strain A6 accumulated 172.0mg/mL 2,5-diketo-D-gluconate in culture medium containing 25% D-glucose and 10% CaCO 3 (W/V) after 7 days’ cultivation at 28℃, with a yield of 52.03 mol%. The glucose tolerance of this strain exceeds that reported in China.更多还原