【摘要】 海藻糖是一种新型食品添加剂 ,目前其主要生产方法是利用微生物酶法合成。文章研究了在不易破壁取得胞内酶的情况下 ,采用渗透处理细胞技术生产透性化细胞酶的方法 ,获得较高酶活力更多还原

【Abstract】 Trehalose is a natural functional disaccharide Trehalose could be widely used in foodstuff, pharmaceuticals, cosmetic, and agriculture Trehalose’s uses are restricted due to the difficulty in its production The permeabilization of Pseudomonas putida cells in relation to trehalose synthase activity was studied using different organic solvents and detergents The performance of these solvents was dependent on the incubation temperature, treatment time, and the concentration of cells Maximum enzyme activity was achieved with 2% toluene and 0 2% EDTA, at 35℃,0 5h The expression of intracellular trehalose synthase activity was increased 117 8 fold with respect to untreated cells更多还原