【摘要】 应用幼年小鼠经孕马血清促性腺激素 (pregnantmare′sserumgonadotropin ,PMSG)处理的动物模型 ,研究了卵泡从发育到闭锁动态变化过程中血管紧张素Ⅱ (AngⅡ )的作用。结果表明 :( 1) 2 4日龄小鼠给予PMSG ( 10IU/只 )后 6d时 ,卵巢中出现大量闭锁卵泡 ,颗粒细胞DNA琼脂糖电泳显示了梯形条带 ;( 2 )随卵泡闭锁发生 ,卵巢AngⅡ含量增加 ;( 3 )AngⅡ显著拮抗FSH刺激颗粒细胞雌二醇生成的作用。我们认为 ,AngⅡ参与了对小鼠卵泡闭锁的调节更多还原

【Abstract】 The effect of angiotensin Ⅱ(Ang Ⅱ) on the follicular development was studied by using an animal model of follicular atresia induced by pregnant mare′s serum gonadotropin (PMSG) The results showed that: (1) a large number of atretic follicles were found in the ovary of 24 day old mouse after 6 day treatment of PMSG Deoxyribonucleic acid (DNA) extracted from granulosa cells clearly showed a ladder band under agarose gel electrophoresis analysis (2) the contents of Ang Ⅱ in the ovary extremely increased with the development of follicular atresia. (3) Ang Ⅱ significantly antagonized the stimulating effect of the follicle stimulating hormone (FSH) on estradiol (E 2) generation of granulosa cells It is suggested that Ang Ⅱ may be involved in the regulation of follicular atresia in mouse更多还原