【摘要】 目的　以 β 谷甾醇为原料制备雄甾 1,4 二烯 3,17 二酮 (ADD)。 方法　利用Mycobacterium phlei932 0 1为出发菌株对 β 谷甾醇进行选择性边链降解制备ADD。对发酵条件如培养基组成、pH和投料时间及转化条件如投料浓度、投料方法和 9α 羟化酶抑制剂等进行摸索以提高ADD产量。结果　当投料浓度为 0 .2 %时 ,转化率达 5 0 %。结论　在 9α 羟化酶抑制剂 2 ,2’ 联吡啶存在时 ,Mycobacterium phlei 932 0 1能有效地将 β 谷甾醇降解为雄甾 1,4 二烯 3,17 二酮更多还原

【Abstract】 Purpose To produce androst-1,4-diene-3,17-dione (ADD) which is a useful intermediated for the synthesis of some difficultly obtainable steroid hormones. Methods We selectively degraded side-chain of β-sitosterol to androst-1,4-diene-3,17-dione by Mycobacterium phlei 93201,investigating the fermentation conditions such as the composition of medium,pH and the time when substrate was added as well as the biotransformation conditions such as the concentration of substrate,the method of substrate added to medium and the optimum concentration of 9α-hydroxylase inhibitor 2,2’-bipyridyl. Results The conversion rate reached about 50% when the concentration of substrate was 0.2%. Conclusions Mycobacte- rium phlei 93201 could degrade β-sitosterol to ADD efficiently in the presence of 9α-hydroxylase inhibitor 2,2’-bipyridyl.更多还原