【摘要】 利用无血清培养基培养角质细胞 ,研究了Ca2 +对鼠角质细胞生长和分化的影响。实验结果表明 ,培养基中钙离子最佳浓度为 0 2mmol L。在此浓度下 ,细胞克隆形成率达到 10 8% ,细胞的贴壁率达到 2 8 7% ,细胞的分化比例和老化比例分别为 5 4%和 2 6 3% ;当Ca2 +浓度达到 0 6mmol L以上时 ,则会引起角质细胞显著的分化和老化更多还原

【Abstract】 The effect of the calcium ion (Ca 2+) on the growth and differentiation of the mouse epidermal keratinocytes cultured in serum-free medium was investigated. It was found that the optimal level of calcium ion in the medium was about 0.2mmol/L. Under such a culture condition the colony forming efficiency, attachment percentage, percentage of the cells with cornified envelops, and percentage of the senesced cells were measured to be about 10.8 %, 30.8 %, 5.1 %, and 26.8 %, respectively. However, the Ca 2+ concentrations in the medium above 0.6mmol/L resulted in significant differentiation and senescence of the keratinocytes, which was found to be harmful for keratinocyte growth and expansion in vitro.更多还原