【摘要】 细胞在模式化表面的定位培养对于组织工程、生物传感器技术和细胞生物学基础研究具有重要意义。研究基于软光刻技术的生物素 -亲和素微模式表面快速制备方法 ,并用所制备的模式进行牛主动脉血管内皮细胞的定位培养。表明该技术可在细胞尺度上有效地控制细胞生长的空间位置更多还原

【Abstract】 The located culture of cells on patterned surfaces is useful for tissue engineering, biosensor development and fundamental research of cell biology. It is presented here a rapid fabrication method of Biotin-Avidin protein layers m icropattern, which is based on soft-lithography technology. The bovine aortic e ndothelial cells are cultured on the micropatterned surface. It is found that ce ll location can be controlled on the scale of individual cell by this method.更多还原