The major antigen region of E 2 gene of Hog Cholera Prevalent Strain (Guangxi Yuling Strain) and Chinese Hog Cholera Lapinised Virus (C-strain) derived from hog and rabbit spleen tissue, was amplified by reverse transcription polymerase chain reaction(RT-PCR) and the nested Polymerase Chain Reaction (nPCR).After the amplified fragments were cloned into the expression vector pP ROEX-HTb, the recombinant plasmids pP ROEX-GXYL and pP ROEX-C were obtained. The insert position ,the size and the readin...
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