【摘要】 水稻齿叶矮缩病毒是一种以水稻为侵害寄主的植物呼肠孤病毒 ,其基因组 1 0条dsRNA编码的蛋白质产物的功能除S9外的大部分还未阐明。报道了该病毒菲律宾分离株S8的开放阅读框序列 ,在大肠杆菌中表达和纯化出了其 6 7kD的蛋白质产物 ,并进一步研究了该产物的功能。实验结果表明 ,S8的蛋白质产物是病毒的一种主要结构蛋白质 ,在体外具有自剪切活性和自聚集性质 ,并推测可能是病毒的内层衣壳蛋白更多还原

【Abstract】 Rice ragged stunt oryzavirus (RRSV) is a member of the genus oryzavirus within the family Reoviridae. Its genome consists of ten segments of dsRNA. The functions of all products encoded by these viral genome segments, except one encoded by S9, have not yet been elucidated. In the present study, the ORF of S8 of RRSV Philippines isolate was sequenced and expressed in E. coli . The 67 kD product of S8 could be self cleaved into two fragments with molecular weights of 43 kD and 26 kD. Western blotting indicated that both 67 kD and 43 kD products were major structural proteins of the virus. It was also found that the 67 kD protein could self aggregate into aggregates with higher sedimentation rate in sucrose gradients during centrifugation. Moreover, the self aggregation process could be accelerated by the complex of S6 product and genome dsRNAs of RRSV. These results suggest that the S8 products, 67 kD or 43 kD, may be the structural components of the viral inner capsid.更多还原