【摘要】 利用3 5S标记的氨基酸混合物喂养工程菌 ,成功地制备了3 5S标记的拟南芥钙调素亚型 2 (3 5S ACaM2 ) ,对其纯度、放射活度、电泳行为及其灵敏性等进行了检测 .结果表明从工程菌中制备的3 5S ACaM 2纯度高、放射活度高、Ca2 + 与EGTA存在时的电泳行为与未标记的ACaM2相同 ,可作为一种高灵敏性的探针用于检测钙调素结合蛋白更多还原

【Abstract】 S ACaM2 was producted by using 35 S labeled amino acid mixture in E.coli . SDS PAGE and autoradiograph indicated that high purified, high specific radioactivity 35 S ACaM2 was obtained.Electrophoresis character of 35 S ACaM2 is the same as that of unlabeled ACaM2 with Ca 2+ or EGTA. Dot blot and NC overlay experiment showed that 35 S ACaM2 could be used to detect calmodulin binding proteins as a sensitive probe.更多还原