【摘要】 发现野生型斜纹夜蛾核型多角体病毒 (Spodopteralituranuclearpolyhedrosisvirus ,SpltNPV)DNA转染SL 1细胞能诱导细胞凋亡 .SpltNPV DNA转染其同源细胞系斜纹夜蛾核SL 1细胞 6h后 ,光镜下即可见细胞膜表面突出或形成小泡 ,细胞碎裂成凋亡小体 ,18h后 ,细胞 10 0 %碎裂成凋亡小体 .DAPI荧光染色显示感染细胞核渐呈半月形 ,直至碎裂被凋亡小体包裹 .被转染的SL 1细胞DNA琼脂糖凝胶电泳呈典型梯形谱带 .野生型SpltNPV病毒粒子感染的SL 1细胞既无多角体的出现 ,也无凋亡现象的发生 .更多还原

【Abstract】 The SpltNPV DNA can induce the apoptosis of SL 1 was discovered. Microscopic examination of Spodoptera litura (SL 1) cell transfected with DNA of Spodoptera litura nucleopolyhedrovirus revealed progressive cell blebbing starting at 6 h postinfection and culminating in total cell destruction at 18 h postinfection. The fragmentation of the infected cell nuclei and apoptotic body were observed by stained with the specific fluorescent dye DAPI. Agarose gel electrophoresis analysis of the DNA extracted from infected cells showed typical DNA ladder. All these supported that the transfected SL 1 cells undergo apoptosis. Neither apoptosis nor polyhedra was observed in the SL 1 infected with the virions of SpltNPV.更多还原