【摘要】 采用 33′ 二巯基硫代丙酸的金电极自组装技术 ,用乙基 3 (3 二甲氨基 )碳二亚胺盐酸盐 (EDC)和N 羟基磺基琥珀酰亚胺 (NHS)偶联剂将亲和素固定于金电极上 ,联于生物素标记的探针 ,制备成压电DNA传感器的检测电极 ,和杂交液中的待检葡萄球菌肠毒素B的ssDNA进行杂交 ,通过频率信号检测DNA杂交的量 ,达到检测的目的 .采用不同长度的基因片段进行了研究 ,制作的传感器一致性、特异性都较好 ;杂交后的电极 ,电极再生后 ,传感器可以重复使用更多还原

【Abstract】 A piezoelectric DNA sensor based on self assembled monolayer technology was developed.In the experiment, 3,3′ dithiopropionic acid was applied to immobilize DNA, 1 ethyl 3 [3 (dimethylamino)propyl] carbodiimide (EDC) and N hydroxysuccinimide (NHS) can coat avidin on gold electrode, and then avidin can combine to different segment (25 mer biotinylated oligonuleotide and 169 bp biotinylated DNA probe by PCR) nucleotide on gold electrode surface. The consistency and speciality of the piezoelectric DNA sensor adopted above immobilization method was well. The sensor can be reused by electrode regeneration.更多还原