【摘要】 用生物标记的方法将色氨酸类似物标记在DsbA蛋白中的色氨酸位置 ,分析标记蛋白质的谱学性质、色氨酸结构环境和潜在应用前景 .5 OH Trp标记的DsbA蛋白具有 315nm激发的荧光发射光谱 ;1 9F NMR能分辨 5 F Trp标记的DsbA蛋白的两个F Trp残基 (Trp76和Trp12 6 ) ,Trp76化学位移变化反映二硫键交换引起的结构转化 .进一步将利用标记蛋白的独特荧光和1 9F NMR性质 ,研究DsbA蛋白的氧化还原及与底物蛋白的结合作用 .更多还原

【Abstract】 Tryptophan analogs were biosynthetically incorporated into the tryptophan sites of DsbA protein to investigate the spectroscopic characteristics, local environments of tryptophans. This technique by using tryptophan analog labeling may be of potential application to studying structure function relationships and protein protein interactions. 5 hydroxytryptophan labeled DsbA protein presents a fluorescence excitation wavelength at 315 nm and an emission near 340 nm. The chemical shifts of Trp76 and Trp126 in 5 fluorotryptophan labeled DsbA were identified in 19 F NMR spectra, and change of the chemical shift of Trp76 can reflect the conformational change of DsbA protein during redox reaction. The future work is to use specific fluorescence and 19 F NMR of labeled DsbA protein to study its redox properties and interaction with substrates.更多还原