节点文献
鹅副粘病毒上海株的分子鉴定
THE GENE IDENTIFICATION OF GOOSE PARAMYXOVIRUS SF02 ISOLATE
【摘要】 对新近分离的鹅副粘病毒SF0 2株采用RT PCR方法 ,扩增部分F基因进行测序 ,其裂解位点的序列为112 R R Q K R F117,与新城疫病毒强毒株的特征相符。通过对其F基因与我国大陆新城疫强毒F4 8E9株、疫苗毒Lasota株比较 ,发现该毒株的F基因已发生了较大的变异 ,而与1999年在我国台湾省流行的新城疫病毒基因Ⅶ型有很高的同源性。
【Abstract】 Partial fusion gene of new goose paramyxovirus (GPV) SF02 isolate was amplified by RT PCR and sequenced.The deduced amino acidsequence of the cleavage site region was 112 R R Q K R F 117 ,matching to virulent(velogenic) Newcastle disease virus. This result correlated with the virulence of the isolate in biological assays. The comparison of F gene of SF02 isolate and the domestic standard velogenic strain NDV, F48E9, had revealed 86.5% homology in nucleotide sequences. The SF02 isolate was closer to genotype Ⅶ NDV strain prevalent in Taiwan, China in 1999.
- 【文献出处】 上海农业学报 ,Acta Agriculturae Shanghai , 编辑部邮箱 ,2002年04期
- 【分类号】S852.65
- 【被引频次】14
- 【下载频次】49