【摘要】 用多种方法培养大鼠胚胎神经干细胞并诱导其分化 ,应用相差显微镜和免疫荧光染色方法对其进行观察和比较。发现在培养液中同时加入bFGF和EGF ,并采用较高的细胞种植密度 ,干细胞增生速度最快。提示 2种神经生长因子对加快干细胞的增生具有协同作用 ,适当提高干细胞的种植密度可加快其增生速度更多还原

【Abstract】 Various methods were used to culture rat embryonic neural stem cells (NSCs). The cells were induced to divide so as to be confirmed as NSCs, and were checked by phase contrast microscopy and immunofluorescence. The results showed that cells grew the fastest when both bFGF and EGF were added into the culture medium. Higher NSCs concentration promoted cell proliferation. It suggests that the two neurotrophic factors have cooperative effects on stimulating cell growth and higher NSCs culture concentration can promote its proliferation.更多还原