【摘要】 为提高BPI2 3 Fcγ1重组抗菌蛋白在原核表达系统中的表达和复性率 ,采用定点突变法改造 pBV BPI60 0 Fcγ1 70 0 重组表达载体 ,转化E .coliDH5α后 ,通过温控诱导表达。结果表明 :①突变后BPI2 3 Fcγ1重组抗菌蛋白表达量比突变前提高约 1 0 % ;②表达时间提前约 1h ;③抗菌活性未受影响 ;④复性率未见显著提高更多还原

【Abstract】 In order to improve the expression and renaturation yield of BPI 23-Fcγ1 bactericidal recombinant protein in Prokaryotic system, pBV-BPI 600-Fcγ1 700 recombinant expression vector was reconstructed by site-directed mutation method. The mutational vector was transformed into the competent Escherichia coli DH5α and expressed by temperature induced method. The results showed the amount of the mutational BPI 23-Fcγ1 protein expression was 10% more than that of the non-mutant one, and its expression time was an hour earlier than the latter. Its bactericidal capability was the same as before, but its renaturation yield was not improved observably.更多还原