【摘要】 目的 :探讨P1 6基因、细胞周期素D1 (cyclinD1 )在子宫内膜癌中的表达及其与临床分期、病理分级的关系。方法 :用免疫组化法检测 41例子宫内膜癌、1 5例增生型子宫内膜、8例正常子宫内膜中P1 6蛋白、cyclinD1蛋白的表达 ,并对比观察其表达在子宫内膜癌不同的病理分级、肌层浸润、临床分期中的差异。结果 :P1 6蛋白在子宫内膜癌中阳性表达率为 5 1 .2 % ,明显低于正常及增生型子宫内膜 (P <0 .0 1 ,P <0 .0 5 )。子宫内膜癌中cyclinD1表达阳性率为 5 6.1 % ,高于正常和增生型子宫内膜 (P <0 .0 1 )。P1 6表达随着子宫内膜肿瘤病理分级、浸润深度和临床分期增高而降低 ,而cyclinD1蛋白表达阳性率却逐渐增高。两者协同表达异常则见于低分化、深肌层浸润及晚期子宫内膜癌。结论 :P1 6基因、cyclinD1的异常表达与子宫内膜癌的发生、发展密切相关 ,可作为指导临床治疗和判断预后的指标更多还原

【Abstract】 Objective: To delve into the expression and clinical significance of the protein of the suppressor gene P16, and oncogene cyclinD1 in human endometrial carcinoma, and to find out the correlation between their expression and clinical stages, pathological grades and the degree of myometrial invasion of endometrial carcinoma. Methods: ABC immunohistochemical assay was used to observe the of expression P16 and cyclinD1 protein in 41 cases of endometrial carcinoma, 15 cases of complex hyperplasia endometrium, and 8 cases of normal endometrium. The differences of expression related to different histological grades, degrees of myometrial invasion and clinical stages of endometrial carcinoma were observed. Results: The positive rate of P16 protein expression in endometrial carcinoma was 51.2%, significantly lower than that in normal endometrium(P<0.01) and complex hyperplasia endometrium(P<0.05). The positive rate of cyclinD1 expression in endometrial carcinoma was 56.1%, which was significantly higher than that in normal endometrium(P<0.01) and complicated hyperplasia endometrium(P<0.01). The expression of P16 gene diminished with higher pathological grades, greater degrees of myometrial invasion and advaneed clinical stages, but the expression of cyclinD1 enhanced. Synchronistic abnormal expression of the two parameters usually correlated with endometrial carcinoma of grade Ⅲ, with deep invasion of myometrium and at stages Ⅲ or Ⅳ. Conclusion: Abnormal expression of P16 gene and cyclinD1 were closely related to occurrence and development of the endometrial carcinoma. They may be ueed as parameters to direct clinical therapy and to evaluate the prognosis of endometrial carcinoma.更多还原