【摘要】 从人肝组织中抽提总RNA,通过RT-PCR方法扩增出人纤溶酶原(plasminogen)Kringle1片段,构建pPIC9K-K1载体,电击转化酵母,成功获得pPIC9K-K1/GS115工程菌.摇瓶发酵初步结果表明,工程菌能稳定表达可溶性的重组K1蛋白,分子量为15kD,发酵液中目的蛋白含量为84.17mg/L.更多还原

【Abstract】 Total RNA was extracted from human fresh liver. The gene encoding Kringle 1 of plasminogen was amplified by RTPCR. Then Kringle 1 gene was introduced into vector pPIC9K. Yeast cell GS115 was transfected by linear recombinant plasmid. Finally the yeast engineering cells named pPIC9KK1/GS115 was obtained stably expressing Kringle 1. Flask test showed that recombination protein K1 in culture supernatants reached up to 84.17 mg/L.更多还原