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中华绒螯蟹血淋巴细胞观察

Observation on hemolymphcytes of Eriocheir sinensis

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【作者】 张凤英王进科

【Author】 Zhang Fengyin,Wang Jinke(210097,Dept of Biology,Nanjing Normal University)//J.Aquaculture -2002.5:30~32

【机构】 南京师范大学生命科学学院水产科学研究所南京师范大学生命科学学院水产科学研究所 210097210097

【摘要】 本文通过显微和亚显微结构的观察研究,表明中华绒螯蟹血淋巴液中存在细胞形态、超微结构不同的三种血淋巴细胞,即大颗粒细胞、小颗粒细胞和无颗粒细胞。血淋巴液中三种淋巴细胞的含量分别为:大颗粒细胞7.97%,小颗粒细胞15.58%,无颗粒细胞76.45%。光镜下大颗粒细胞体积最大,胞质内可见大量染色较深的粗大颗粒;小颗粒细胞体积较小,胞质内有较少的深染色细小颗粒;而无颗粒细胞体积最小,胞质内无颗粒。电镜下三种血淋巴细胞的结构特征表现为:大颗粒细胞胞质内含有大量粗大的电子致密颗粒,细胞器稀少;小颗粒细胞胞质内含数量较少、体积较小的电子致密颗粒,细胞器丰富,高尔基复合体可见;无颗粒细胞内无电子致密颗粒,细胞器稀少,部分细胞质接近透明。

【Abstract】 The structure and ultrastructure of hemolymphcytes of Eriocheir sinensis were studied in this paper.Accordin g to the morphology,ultrastructure and granules in the c ytoplasm,the hemolymphcytes were classified into three distinguished kinds,which were large -gran-ular,small -granular and non -granular cell.The quantities of large -granula rcell.smali -granular cell and non -granular cell in hemolymph were respectively 7.97%,15.58%and 76.45%.Through light mic roscope,the three kinds of blood cells were di stinct in their structure.Large -granular cells co ntained larger mount of crude granul es and small -granular cells contain ed fewer smaller granules,but non -granular cells contained no gra nules.Under transmission electron microscope,large -granular cells were the largest and contained many big electron -dense granules an d a few cellular organs,small -granular cells had a few smaller granules with plentiful cellular or-gans including Golgi complex,and non -granular cells were smaller than the two former without electron-dense granules.

  • 【文献出处】 水产养殖 ,Journal of Aquaculture , 编辑部邮箱 ,2002年05期
  • 【分类号】S968.25
  • 【被引频次】7
  • 【下载频次】127
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