【摘要】 作者研究的目的是建立一种较为灵敏、简便的方法检测环氧合酶COX 2 .先设计了一对针对COX 2的特异性引物 ,建立了以半定量逆转录 聚合酶链反应 (Semi QuantitativeReverseTranscrip tionPCR ,SQRT PCR)方法检测COX 2的mRNA ,用该方法检测 7种结肠癌细胞和非甾体类抗炎药吲哚美辛 ,作用于其中一种细胞后的COX 2mRNA .实验结果显示 ,半定量RT PCR法可以灵敏、快速地反映COX 2mRNA表达水平的变化 ,为寻找新的COX 2选择性抑制剂和分析NSAIDs抗炎作用机理提供了一种新的方法 .更多还原

【Abstract】 The aim of this study is to establish a sensitive and easy method to detect cyclooxygenase-2 (COX-2). The authors designed a pair of gene specific primers for COX-2, then established a method to investigate the expression of COX-2 with semi-quantitative reverse transcription PCR (SQ RT-PCR). By using this method, the authors tested seven colon cancer cell lines and one of them was treated with Indomethacin. The experiment results showed that SQ RT-PCR can detect the change of COX-2 mRNA quickly and sensitively. It will be a good and useful method in seeking selective COX-2 inhibitors and studying the mechanism of Nonsteroidal antiinflammatory drugs (NSAIDs).更多还原