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四川绵阳直立和匍匐麦冬的核型分析

Karyotypical Analysis of Erective and Creepy Ophipogonjaponicus in Mianyang of Sichuan

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【作者】 曹毅乔代蓉郑鸣邢红霞蒋彦

【Author】 CAO Yi 1,QIAO Dai-rong 1,LI Yan-xi 1,XING Hong-xia 1,JIANG Yan 2 (1.National Lab for Grassland Biotechnology,Sichuan University,Chengdu 610064,China; 2.Sichuan Agreculture University,Sichuan Yaan 625000,China)

【机构】 四川大学草原生物工程国家实验室四川农业大学 成都610064成都610064四川雅安625000

【摘要】 采用低渗去壁法对四川绵阳直立和匍匐两种麦冬进行核型分析和染色体计数 直立和匍匐麦冬色体数目均为 2n =6 8,染色体基数x =17 最长与最短染色体的相对长度分别为3.35和 3.0 8.

【Abstract】 In order to open out the genetic essence of standard Ophiopogon japonicus in Mianyang,the karyotype of two kinds of them were compard.The method of low osmosis was adapted to eliminate cell walls of O.japonicus’s root point.The results show that it was better to get karyotype of O.japonicus selecting the root point at 9∶00~9∶00 and using 25% mixed solution of pectinase and cellulase to digest cell walls for 5h.The chromosome numbers of erective and creepy O.japonicus in Mianyang were 2n=68 and its cardinal number of chromosome was x=17.The relative length of chromosomes of the two O.japonicus was 3.35 and 3.08,respectively.

【关键词】 麦冬染色体核型
【Key words】 Ophiopogon japonicuschromosomekaryotype
【基金】 四川省重点项目基金
  • 【文献出处】 四川大学学报(自然科学版) ,Journal of Sichuan University (Natural Science Edition) , 编辑部邮箱 ,2002年02期
  • 【分类号】Q949.7
  • 【被引频次】15
  • 【下载频次】179
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