【摘要】 用高效液相色谱法对水化油脚提取物中所得的卵磷脂(PC)进行了定性和定量分析,采用Lichorosorb si-60色谱柱,氯仿-甲醇-水为流动相,蒸发光散射检测器检测。结果表明:在15min内可将卵磷脂与其它组分完全分离,用外标法进行定量,线性关系良好。卵磷脂的平均回收率为97.69%～99.23%,相对标准偏差为0.82%～0.94%。更多还原

【Abstract】 The phosphatidylcholine in hydration oil leftover was determined qualitatively and quantitatively by HPLC with Lichorosorb si-60 chromatographic column, flow-phase of chloroform-methanol-water and evaporate-scattering detector. The result showed: the PC was separated completely with other components in twelve minutes. The quantitative analysis of the PC has been achieved with external standard method. The linear relation is good. The average recovery of PC was 97.69～99.23%,the relative standard deviation was 0.82～0.94%.更多还原