【摘要】 应用水稻(Oryza saliva)2 200条非冗余表达序列标签(EST)所制备的eDNA微阵列及同位素33P杂交体系,对水稻抗白叶枯菌近等基因系中早14R、中早9S经白叶枯病菌(Xanthomonas oryzae pv.oyzae(Xoo))诱导后特异基因表达模式进行研究。结果表明:四叶期诱导3 h后,在1 336对有效数据所代表的基因中表达丰度上调有315个,下调有176个。对其中78个受诱导的已知功能基因进一步分析表明:cDNA微阵列上基因表达变化反应了植物防卫反应中基因表达的协同作用以及对病原物的攻击作出防卫反应的基本模式;尽管以往研究中一些基因未明确与植物抗病有直接关系,但在供试材料受白叶枯菌诱导后却发现其表达量发生了明显改变,对此作了初步讨论;另外,实验结果还发现了多个受白叶枯菌强烈诱导但功能未知的相关基因。更多还原

【Abstract】 The expression profiles of rice (Oryza sativa ) near isogenic line (NIL) Zhongzaol4R (resistance) and Zhongzao 9S (susceptible) in response to Xanthomonas oryzae pv. oyzae (Xoo) were studied using cDNA microarray containing 2 200 expression sequence tags (ESTs) and 33P hybridization. Results showed that the expression of 315 genes were up-regulated while 176 genes down-regulated among the valid data of 1 336 genes after induced for 3 h with Xoo at 4-leaves stage. Further studies of the 78 induced genes with known functions indicated that the changes of gene expression pattern not only reflected coordinating gene regulation in plant defense but also showed the basic model of plant in response to pathogen attack. Some genes never reported to related to the plant defense response while induced by Xoo were reported here by microarray analysis. Novel genes induced by Xoo were also found by using this high-throughput cDNA microarray method.更多还原