【摘要】 苏云金芽孢杆菌(Bacillusthuringiensis,Bt)毒素蛋白由3个不连续的结构域(domains)组成,其中结构域Ⅰ为膜跨越区,与膜孔道形成有关。结构域Ⅱ、Ⅲ与受体结合有关。利用基因重组技术对鳞翅目昆虫具专一活性的毒素蛋白Cry1Aa与专一性有关的结构域编码区与对鞘翅目昆虫具专一活性的毒素蛋白Cry3A编码基因融合,构建成融合表达载体,为进一步构建Bt工程菌和Bt转基因植物奠定基础。更多还原

【Abstract】 Bacillus thuringiensis (Bt) pesticidal crystal protein possesses three separate domains. Domain Ⅰ might be responsible for toxicity and Domain Ⅱ andⅢ for specificity. In this study, a DNA fragment encoding the Domain Ⅱ andⅢ of Cry1Aa toxin was fused with Cry3A toxin protein. An expression plasmid with the fusion protein was also created for further research relating to genetic engineered Bt and transgenic crops.更多还原