【摘要】 应用免疫组织化学方法研究了血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)及其受体在大鼠(Spraguedawley )子宫中的定位。结果显示:①在动情周期的大鼠子宫中AngⅡ免疫阳性物主要定位于子宫腺、腔上皮,其免疫染色强度在动情前期最高,间情期最低。②围着床期大鼠子宫中存在AngⅡ、AngⅡtypeⅠreceptor(AT1R)和AngⅡtypeⅡreceptor(AT2R),亦主要定位于腺上皮和腔上皮,其染色强度随妊娠延长而增加。③妊娠第1天注射RU486的大鼠子宫中AngⅡ,AT1R和AT2R的免疫阳性染色部位没有变化;但AngⅡ及AT2R染色强度增加,而AT1R染色强度降低。AngⅡ及其两种主要受体在大鼠动情周期、围着床期及用RU486抗着床后的子宫内膜中发生相应变化,推测AngⅡ及其受体可能参与了大鼠动情周期与胚胎植入过程的调节。更多还原

【Abstract】 The localization of angiotensinⅡ(AngⅡ)and its two type receptors was investigated by immunochemistry in rat (Sprague dawley )uterus. The results showed that:① Immunoactive AngⅡ was mainly distributed at luminal and glandular epithelium in rat uterus during estrus cycle, and its density was highest at proestrus, while lowest at diestrus; ② Positive staining AngⅡ, AngⅡ typeⅠreceptor (AT1R) and AngⅡ typeⅡ receptor(AT2R) were localized in luminal and glandular epithelium during peri-implantation, their density was increased with the advance of pregnancy; ③The localization of AngⅡ, AT1R and AT2R was not changed in rat uterus treated by RU486, the density of Ang Ⅱ and AT2R was increased after treatment, while AT1R was decreased. These results suggested that AngⅡ and its receptor might be involved in estrus cycle and embryo implantation of rats; and the expression of AngⅡ , AT1R and AT2R was regulated by estrogen and progesterone.更多还原