【摘要】 利用RAPD和ISSR两种分子标记技术对37份甜瓜(CucumismeloL.)种质进行了遗传多样性研究。在供试材料中筛选到具有多态性的RAPD引物21个,ISSR引物10个(其中ISSR-2与ISSR-4等量混合组成ISSR-10引物对)。RAPD引物共扩增出多态性带106条,多态性条带比率(PPB)为58.62%,平均多态信息量(PIC)为0.47;ISSR引物共扩增出多态性带73条,PPB值为65.51%,平均PIC值为0.53。根据两种标记的结果,采用UPGMA聚类分析,将供试材料分为两大类群:野生甜瓜和栽培甜瓜;栽培甜瓜又分为厚皮甜瓜和薄皮甜瓜两大亚群,各野生甜瓜种质之间的遗传距离较大,这与其分类地位基本一致。两种分子标记的分析结果呈极显著正相关(r=0.62﹥r=0.01)。研究表明,RAPD和ISSR标记可用于甜瓜种质遗传多样性的研究。更多还原

【Abstract】 Random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) molecular markers were used to detect the genetic diversity among 37 melon (Cucumis melo L.) germplasms. A total of 21 RAPD primers and 10 ISSR primers (ISSR-10 primer was evenly composed of ISSR-2 and ISSR-4) were identified with polymorphism among the entries. For RAPD markers, 106 polymorphic bands were produced, percentage of polymorphic bands (PPB ) was 58.62%, mean polymorphism information content (PIC ) was 0.47. For ISSR markers,73 polymorphic bands were generated, PPB was 65.51%, mean PIC was 0.53. UPGMA cluster analysis based on two molecular markers data divided these melons into two groups: wild melon group and cultivated melon group. The genetic distance of each wild melon germplasms was far. Cultivated melon group was classified into two sub-groups, which were thick-peel melon and thin-peel melon. They were consistent with the grouping based on taxology systematic botany. Genetic similarity matrices revealed that the estimates of correlation coefficients of RAPD and ISSR were significantly correlated. Based on the results, RAPD and ISSR could be applied to detect genetic diversity analysis among melon germplasm.更多还原