【摘要】 以来自河北、江苏两地的22株小麦叶锈菌作供试菌株,用27个小麦抗叶锈近等基因系品系作为鉴别寄主进行毒性测定。毒性多态性分析结果为:22个菌株通过聚类被分为两组,第一组17个菌株主要来自河北,第二组5个菌株全部来自江苏,表明毒性与地理来源密切相关;菌株间遗传相似系数较高并且差别不大,为0.6296～0.9259之间,说明两地的小麦叶锈菌所含的毒性基因差异不大。应用RAPD技术,从65个随机引物中筛选了12个扩增多态性较好的随机引物,用于分子多态性分析,共扩增出DNA条带102条,其中多态性条带54条。RAPD分析结果为:22个菌株被分为两组,第一组菌株主要来自河北,第二组菌株主要来自江苏,说明DNA多态性与地理来源间具有一定的相关性;菌系间遗传相似系数相差较大,为0.3889～0.9074之间,说明两地小麦叶锈菌群体遗传结构丰富而复杂。比较22株小麦叶锈菌在27个鉴别品系上的毒性特征和54个RAPD标记建立的聚类分析树状图,发现以RAPD标记为基础的分子多态性与毒性多态性相关性不强。更多还原

【Abstract】 22 strains of Puccinia recondite f.sp tritici collected from Hebei and Jiangsu were analyzed.Their virulence were tested on 27 near-isogenic wheat differential lines.The results of dendrogram analysis showed that strains can be devided into 2 groups,the first group including 17 strains was mainly from Hebei province,and 5 strians of the second group were all from Jiangsu province, which showed close relationship between virulence and source;The differencs between similarity coefficients of strains are smaller (0.6296~0.9259), it means small difference on virulence genes contained in Puccinia recondita f.sp tritici from Hebei and Jiangsu.22 strains were analyzed by RAPD technology, 12 from 65 random primers were effective to produce DNA polymorphic bands.There were 54 polymorphic bands among the total 102 bands of RAPD.The results of dendrogram analysis showed that the 22 strains were classified into two groups. Strains contained in the two groups mainly come from Hebei and Jiangsu respectively,which means some relationship between DNA polymorphism and source.The difference between similarity coefficients of strains were larger (0.3889~ 0.9074),which shows that of Puccinia recondita f.sp tritici were genetically complex.Compared Virulence with was DNA polymorphism,there was no close relationship between virulence and DNA polymorphism.更多还原