【摘要】 橄榄绿链霉菌A1（Streptomyces olivaceoviridis A1）所产木聚糖酶XYNA经阴离子交换层析和分子筛层析分离,得到纯化的XYNA。XYNA的分子量约为43kD。其最适温度为60℃,最适pH5.6。XYNA在55℃下以4-O-Me-D-glucurono-D-xylan的Km值和Vmax分别是3.32g/kg和15.72μmol（mL·min）。SDS、EDTA对XYNA有轻微的抑制作用。XYNA无纤维素酶活性。胃蛋白酶、胰蛋白酶处理30min,对酶活性无影响。XYNA成熟蛋白N端10个氨基酸序列为Ala-Glu-Ser-Leu-Gly-Ala-Ala-Ala-Ala。更多还原

【Abstract】 Xylanase XYNA produced by Streptomyces olivaceoviridis A1 was successively purified by anion exchange chromatography, size chromatography. The molecule weight of the XYNA was determined as about 43kD by SDS-PAGE. Optimal pH Value and temperature of XYNA for its activity were 5.6 and 60℃ .respectively.The Km Values and Vmax of XYNA for 4-O-Me-D-glucurono-D-xylan under 55℃ were 3.32g/kg and 15.72μmol/ (mL·min),respectively.The activity of XYNA could be decreased slighly by SDS and EDTA. The XYNA didn’t display the activity of cellulase. Treated by pepsin or trypsin for 30 min under 37℃,the activity of XYNA wasn’t effected.The N-terminal amino acids sequence of XYNA protein was determined as Ala-Glu-Ser-Thr-Leu-Gly-Ala-Ala-Ala-Ala by amino acids sequence analysis.更多还原