【摘要】 为了用分子生物学方法对油桃品种进行分析 ,本研究利用 RAPD技术 ,采用从 2 0 0个 1 0碱基随机引物筛选的 2 2个引物对 1 9个油桃品种的基因组 DNA进行扩增 ,通过扩增 1 80个位点谱带的聚类 ,分析供试油桃的系统发育 ,将供试油桃品种分为 3类 ;并运用特殊谱带 ,建立了油桃的品种分子识别表 ,提出了重点保存的油桃种质有曙光、瑞光 2号、五月火、艾米拉、红李光、阿姆肯更多还原

【Abstract】 To analyze nectarine cultivars from angle of molecular biology, genomic DNAs of 19 accessions of nectarine peach ( Amgdalus persica var. necturina ) were amplified by RAPD (Randomly Amplified Polymorphic DNA) with 22 primers selected from 200 arbitrary 10 decamer primers. Analyzing phylogenetic relationship through cluster of the bands of 180 amplified loci. The experimented accessions could be divided among three groups. Molecular checking index and conservation of key germplasm which included 6 cultivars named Shuguang, Ruiguang No.2, Mayfire, Aimila, Hongliguang, Armking were put forward according to special bands.更多还原