【摘要】 目的　探讨宁夏固原地区结核分子流行病学的特点。方法　提取结核分枝杆菌基因组DNA ,经限制性内切酶PvuⅡ切割、琼脂糖凝胶电泳和Southern转印后 ,用荧光标记的IS6 110DNA序列中 2 45bp探针杂交 ,以核酸化学发光试剂盒探测荧光信号 ,比较各菌株指纹的IS6 110拷贝数和带型 ,分析流行菌株的特点。结果　固原地区流行结核分枝杆菌临床分离株DNA指纹图谱带型相似 ,IS6 110拷贝数较多。结论　固原地区流行的结核分枝杆菌多态性程度较低 ,在基因上具较近的亲缘关系。更多还原

【Abstract】 Objectives To study moleculer epidemiology of the strains of mycobacterium tuberculosis(MTB) isolated from Guyuan.Methods Chromosomal DNA of MTB was digested with endonuclease PvuII, then electrophresed on agarose gel plate and transferred capillarilly to a nylon filter and hybridized with 245bp fragment of IS6110 which labeled using random primer fluorescein labeling kit.The RFLP patterns of Southern hybridization were inspected autofluorographically and the chromosomal DNAs of MTB were thereby typed.Results The strains of MTB isolated from Guyuan carry 10-22 copies,which are present in a less variety of chromosomal site.Conclusions There is low genotypic variability in Guyuan isolates.更多还原