【摘要】 目的　研究吐温 - 80合并温热对BT - 32 5人多型性胶质母细胞瘤细胞系BT - 32 5细胞的分化诱导效应。方法应用免疫细胞化学等方法 ,观察BT - 32 5细胞经吐温 - 80合并 4 1℃温热作用后 (2 4小时 )胶质纤维酸性蛋白 (GFAP)基因的表达 ;并通过克隆形成率及测定细胞生长曲线等方法观察细胞恶性增殖能力。结果　 (1)BT - 32 5细胞经吐温 - 80合并温热作用后 ,出现明显的分化特征 :胞体显著增大 ,贴壁明显 ,细胞突起增多 ,与周围细胞接触 ,且有接触抑制现象 ;GFAP的含量明显增加 ;(2 )克隆形成率显示合并作用后克隆形成数减少 ,与对照组比较有显著差异 (P <0 .0 1)。结论　吐温 - 80合并 4 1℃温热作用可显著抑制BT - 32 5细胞恶性增殖 ,并有促进GFAP表达的效应 ,显示吐温 - 80合并温热对BT - 32 5细胞的分化有诱导效应更多还原

【Abstract】 Objectives To study the differentiation induced effects of Tween-80 combined with hyperthermia in human glioblastoma multiforme BT-325.Methods Tween-80 in combination with hyperthermia(41℃) was administered to the BT-325 cells. The protein expression levels of GFAP was assayed by the immuno-cytochemistry. The malignant proliferation of BT-325 cells were measured by cloning efficiency and cell growth curve.Results That combined with Tween-80, hyperthermia 41℃could dramatically increase the expressions of GFAP (P<0.01) and the differentiation characters could be observed distinctively,such as the increased size and the marked increase in cell process formation and cell-to-cell connection.The size and the cloning efficiency reduced obviously (P<0.01).Conclusions The results demonstrated that Tween-80 in combination with hyperthermia(41℃) could significantly inhibit proliferation, decrease the degree of malignancy and increase the expressions of GFAP. It could induce the differentiation of BT-325 cells.更多还原