【摘要】 目的 :观察维生素 C与三氧化二砷相互联用时 K5 6 2细胞株凋亡率的变化。方法 :联用不同浓度的维生素 C与三氧化二砷孵育 K5 6 2细胞株不同时间 ,采用电镜观察及流式细胞术通过 PI染色检测在两种药物单用及联用情况下的细胞凋亡比例。结果 :维生素 C与三氧化二砷联用组细胞凋亡率明显高于单用三氧化二砷组。结论 :与维生素 C联用可明显提高三氧化二砷对 K5 6 2细胞株凋亡诱导作用 ,与低剂量 1μmol/ L三氧化二砷联用时其凋亡率甚至明显高于单用 5 μmol/ L三氧化二砷组。为临床上砷剂对急性非淋巴细胞性白血病治疗的应用提供了新思路更多还原

【Abstract】 Objective:To explore the apoptosis rate of cell line K562 induced by combined arsenic trioxide with ascorbic acid for clinical application.Method:K562 cells were treated with different concentration of ascorbic acid and arsenic trioxide for different time, then the apoptosis of K562 cells was detected by transmission electron microscope and analyzed by flow cytometry with prodium iodide.Results:Combined ascorbic acid with arsenic trioxide will evaluate the apoptosis rate of K562 markedly. Conclusion:Combination of arsenic trioxide with ascorbic acid will increase apoptosis rate of K562 cells markedly. It might provide theoretical basis for a new clinical application of arsenic trioxide.更多还原