【摘要】 通过逆转录 PCR从无花果曲霉总 RNA中扩增出 1 .4 -kb带信号肽的植酸酶基因 ,将其克隆到穿梭表达载体 p YES2 ,构建了含有目的基因的重组质粒 p YPA1 .用 p YPA1转化酿酒酵母 INVSc1菌株 ,成功地构建了能分泌活性植酸酶的工程菌株 .该菌株的成功构建为饲料和食品添加剂以及解磷工程菌肥的开发奠定了基础 .更多还原

【Abstract】 A 1.4 kb phytase gene amplified by RT PCR from A.ficuum total RNA was successfully cloned into the expression vector pYES2, subsequently the recombinant expression vector pYPA1 was obtained. By the transformation with pYPA1, a transgenic S. cerevisiae that could express extracellular functional phytase was constructed. The successful construction of this engineering yeast strain made the groundwork for research on feed additive and microbial fertilizer.$$$$更多还原