【摘要】 目的:研究巨噬细胞集落刺激因子(M-CSF)和白细胞介素(IL)-10对人外周血单核细胞肿瘤坏死因子-α(TNF-α)和IL-8的诱生作用及相互影响。方法:自健康献血员血液中分离单核细胞进行体外培养,并以M-CSF、IL-10单独及共同作用,然后收集上清,以双抗体夹心酶联免疫吸附试验(ELISA)法和生物活性法测TNF-α,以ELISA法检测IL-8。结果:①M-CSF能诱导单核细胞分泌TNF-α(P<0.05),与脂多糖(LPS)具有诱生TNF-α的协同效应(P<0.05);IL-10则能抑制TNF-α的产生(P<0.05),并拮抗M-CSF增强LPS诱生TNF-α的作用(P<0.05);②M-CSF能诱导单核细胞分泌IL-8(P<0.05),IL-10则能抑制IL-8的分泌(P<0.01),并拮抗M-CSF对IL-8的诱生效应(P<0.05)。结论:M-CSF可以促进单核细胞释放炎性细胞因子,是体内重要的炎症性因子之一,而IL-10则是体内重要的炎症抑制因子,能拮抗M-CSF的致炎作用。更多还原

【Abstract】 Objective:To investigate the interaction between recombinant human macrophage colony stimulating factor(rhM CSF)and interleukin(IL) 10 on TNF α and IL 8 generated by human peripheral blood monocytes.Methods:Human monocytes,isolated from the blood of healthy donors,were cultured with M CSF or IL 10 alone,or combined M CSF with IL 10.Seventy two hours later TNF α in culture supernatans were determined by ELISA and the method of biological activity detection, and IL 8 in culture supernatans were detected by ELISA 48h after culture. Results:1 M CSF was able to induce generation of TNF α by monocytes and had a synergetic effect with LPS in induction of TNF α.While IL 10 was capable to inhibit production of TNF α and had an antagonism to induction of TNF α by LPS and M CSF.2 M CSF had a capability to induce secretion of IL 8 by monocytes。While IL 10 could suppress secretion of IL 8 and had an antagonism to induction of IL 8 by M CSF.Conclusion: M CSF may promote monocytes to release inflammatory cytokines and plays an important role in inflammatory reaction in vivo;while IL 10,as a dramatic inflammation inhibitor, may have an antagonism to in flammatory effect of M CSF.更多还原